Proteomic Changes during the Dermal Toxicity Induced by Nemopilema nomurai Jellyfish Venom in HaCaT Human Keratinocyte

Jellyfish venom is well known for its local skin toxicities and various lethal accidents. The main symptoms of jellyfish envenomation include lesions, burning, prickling, stinging pain, red, brown, or purplish tracks on the skin, itching, swelling, leading to dermonecrosis scar formation. However, molecular mechanism behind action human cells rarely understood. In present study, we have treated HaCaT keratinocyte with Nemopilema nomurai (NnV) study detailed mechanisms actions after envenomation. Using two-dimensional gel electrophoresis (2-DE) matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF/MS), cellular changes at proteome level were examined. treatment NnV resulted in decrease cell viability a concentration-dependent manner. (at IC50), alterations determined 12 h 24 treatment. Briefly, 70 protein spots significant quantitative picked from gels MALDI-TOF/MS. total, 44 differentially abundant proteins successfully identified, among which 19 increased, whereas 25 decreased abundance levels comparing their respective control spots. DAPs involved survival development (e.g., Plasminogen, Vinculin, EMILIN-1, Basonuclin2, Focal adhesion kinase 1, FAM83B, Peroxisome proliferator-activated receptor-gamma co-activator 1-alpha) expression, stress immune response-related Toll-like receptor 4, Aminopeptidase N, MKL/Myocardin-like hypoxia up-regulated Heat shock 105 kDa, Ephrin type-A some protease (or peptidase) enzymes) up-regulated. conclusion, findings may exhibit possible key players during damage suggest therapeutic strategies preventing